Departments

Missions

The Resources technologies service is in charge of internal research and development programs. The main goal of this service is to create new mouse tools for the community.

Resources

CreERT2 zoo : database of more than 50 cell or tissue specific promoters driven CreERT2 transgenic lines.
NR Zoo : database of the nuclear receptors and there co-factors developed as mouse conditional knock-out models.
MousePat : database providing comprehensive analysis of the Nuclear Receptors (NRs) expression in the adult mouse brain.

Techniques

qPCR, RT-qPCR
lacZ staining
in situ hybridization
Immunohistochemistry

New tools

Highly-efficient, fluorescent, locus directed Cre and FlpO deleter mice on a pure C57BL/6N genetic background

In order to facilitate the use of the new mutant resources developed in the mouse, we have generated Cre and FlpO deleter mice on a pure inbred C57BL/6N background. The new transgenic constructs were designed to drive either the Cre or FlpO recombinase, fused to a specific fluorescent marker (respectively the eGFP or the eYFP) and were inserted by homologous recombination in the neutral Rosa26 locus. They allow a rapid, cost-effective and efficient identification of the carrier individuals through the co-expression of the fluorescent marker.

The main advantages of these deleters are:

Preservation of a pure genetic background when used to remove specific selection cassette or to induce complete loss-of-function allele (pure inbred C57BL/6N background)
Insertion in the Rosa26 locus
Simplicity of genotype identification (fluorescence evaluation)
High and stable recombination efficiency (up to 100%)
Expression of cre and FlpO in developing oocytes irrespectively of the transmission of the Cre and FlpO transgenes

Publication

Request the FlpO deleter: Gt(ROSA)26Sor<tm2(CAG-flpo,-EYFP)Ics (EM:05490)

Request the Cre deleter: Gt(ROSA)26Sor<tm1(ACTB-cre,-EGFP)Ics (EM:05488)