Resources and Technologies

To secure and to control the huge amount of data generated by all the operations, a Laboratory Information Management System (LIMS) has been developed. The ICS has a dedicated team of 4 people to manage its information system. The team has developed modern, web-based applications to capture process and analyse all relevant data of the ICS. These databases cover major activities (genetic engineering, animal facility, phenotyping), as well as transversal fields such as quality control.

The team has published several public resources, such as MousePat, NR Zoo and CreER^T2 Zoo, and plans to provide more in the future in order to create new mouse tools for the community.
Jointly with the IGBMC, the team has also developed a strong expertise in statistics and data mining. In-depth analysis performed across studies allows for a better understanding of systems biology and optimized processes.
The team has a strong expertise in collaborations with partners from different origins. It participates actively in European projects, such as EUMODIC, and helps in the design of its database Europhenome, as well as in the set up of the international IMPC program database. Interactions with big pharmaceutical companies have also been successfully established to deliver in time scientific projects.

ICS resources

Genetic Engineering Mouse Zoo

NR Zoo
- Database of the nuclear receptors and their co-factors developed as mouse conditional knock-out models.
- These mouse lines are available for academic users under MTA agreement and distribution fees.
EUCOMM Lines
- Generation, archiving, and world-wide distribution of up to 12.000 conditional mutations across the mouse genome in mouse embryonic stem (ES) cells
- Establishment of a limited number of mouse mutants from this resource
PHENOMIN Lines
- 235 new mouse lines are now available and 31 more are in the process of being generated. Informations regarding the selected projects can be found in the genes table.
- More than 50 mouse models for rare diseases.

CreER^T2 Zoo

MouseCre
- Database of more than 50 cell or tissue specific promoters driven CreER^T2 transgenic lines.

MousePat

MousePat
- Database providing comprehensive analysis of the Nuclear Receptors (NRs) expression in the adult mouse brain.

Distribution fees

Live Mice (6-7 weeks of age, 2-3 Breeding pairs): 3200 €
Frozen materials: 1600 €

In most of the case only heterozygous carrier has been used for archiving and thus the distributed materiel may contain carrier (mutant or transgenic) and control (not mutant or not transgenic) embryos or gametes. ICS offers additional breeding services to e.g. generate cohorts of homozygous mutants. A quotation for such a service is available upon request.

Please note that all the lines are not specifically maintained for distribution. We thus cannot guaranty immediate shipment of live animals you requested. Once a request of interest is received, you will be contacted by email within a week to give you an up-to date availability of the requested line.

More Details on the restrictions, on PI and on the distribution is available upon request.

FAQs CRISPR Cas9

CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models. Now we can offer the generation of knock-out models though direct injection into fertilized mouse oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.

Download the FAQs in PHENOMIN's resources web page: CRISPR guidelines.