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Genotyping is often considered as a straightforward and easy step; in reality providing robust, accurate, and fast results is frequently more challenging that it seems.

Objectives: 

• To be able to define a genotyping strategy and understand its interest in the analysis of its model (rodent, zebrafish)
• Be able to design a genotyping strategy taking into account genetic modification
• Know how to choose an analysis method according to the allele to be identified
• Be able to optimise the design of the strategy in terms of primer selection criteria, amplicon size and structure and the development of experimental conditions
• Be aware of the impact of genotyping error