Departments

Head

Marie-France Champy

Scientific advisors

• Gérard Gradwohl
• Jocelyn Laporte
• Daniel Metzger

Staff

3 persons

Missions

The metabolic exploration platform of ICS performs specific, on-demand experiments as well as comprehensive metabolic analyses with primary and secondary phenotypic screens to evaluate in vivo the energy balance in mouse. These screens are performed under either basal condition or energy challenges using standardized techniques for the detection of phenotype in energy metabolism, glucose homeostasis, intestinal function, bone metabolism and renal function. It provides users with the techniques and scientific support used to design experimental protocols as well as for the development of new techniques to investigate metabolism. The metabolic exploration platform of ICS has a large expertise in phenotyping mouse model for diabetes, obesity and atherosclerosis.

Tests

• Energy metabolism
  • Growth rate and body weight progression
  • Dysmorphology screen
  • Body composition
  • Food intake
  • Energy expenditure
• Glucose homeostasis
  • Glucose tolerance test
  • Insulin sensitivity test
  • Hyperinsulinemic euglycaemic clamp
• Bone metabolism
  • X-Ray analysis of the skeleton
  • Bone mineral density
  • Micron-scale Computed Tomography (µCT) for bone architecture
• Intestinal function
  • Metabolic and diuresis cages
  • Bomb calorimeter: evaluation of gross energy in feces
• Renal function
  • Metabolic and diuresis cages
  • Spot urine
  • Urine chemistry and protein excretion
• Challenges / Models
  • Exercise test
  • Cold exposure
  • Food restriction and refeeding
  • Diet-induced obesity model (DIO)
  • Atherosclerotic model
  • Inflammatory disease models ("NEW")
  • Wound healing ("NEW")

The metabolic analysis of the mice could be combined with clinical assessments, by performing biochemical analysis in blood, plasma, urine and tissues (i.e, lipid and cholesterol content, glycogen content...) and by performing autopsy of the animal at the end of phenotyping study with several tissue collections (i.e, histology, mRNA anlaysis, protein analysis...).