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A conditional Knock-Out allows the deletion of a gene in a tissue -and/or time- specific manner.
If the recombinase is expressed in the fertilized embryo, the generated model will be a full Knock-Out.
If the recombinase is expressed only in a population of cells, the generated model will be a "tissue-specific" Knock-Out.

1. Site-specific recombination sites are introduced into intronic sequences surrounding key exons.
2. The resulting sequence corresponds to the conditional allele.
3. This allele is designed so that a wild type transcript is produced.
4. After recombination with a recombinase, the allele is deleted resulting in a Knock-Out.

Step 1
Step 2
Step 3
Step 4

ICS expertise:

Large ES resource already available (EUCOMM; KOMP;NorCOMM). As a member of the EUCOMM consortium, the ICS has a strong experience using the EUCOMM and KOMP resources.
Our know-how in designing these models will reduce the risk that the conditional allele does not behave as the endogenous wild type allele.
This strategy is specially adapted for indispensable genes and for the study of the function of a gene in a specific biological system, organ or cell population. We recommend also this strategy for gene with unknown function(s).

Risks:

Higher risk of production of truncated / partially functional protein after deletion of the conditional allele than a constitutive Knock-Out approach.
The size of the inter-LoxP fragment is limited; it can sorely exceed 3 kb as homologous recombination can occur between the two loxP sites.

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1. Site-specific recombination sites are introduced into intronic sequences surrounding key exons.

2. The resulting sequence corresponds to the conditional allele.

3. This allele is designed so that a wild type transcript is produced.

4. After recombination with a recombinase, the allele is deleted resulting in a Knock-Out.

ICS expertise:

Risks: