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The tail flick test measures a simple spinal reflex to a
sudden painful thermal stimulus.
A photobeam is used to apply a heat stimulus to the tail, and the time to flick
the tail out of the path of the light beam is measured.
Tail flick equipment (Panlab, Barcelona, Spain)
10 mice per group are recommended for reliable data analysis.
The hot plate test is used for evaluation of response to acute
thermal pain.
The mice are placed into a glass cylinder on a hot plate adjusted to 52°C and
the latency of the pain reaction (licking, moving the paws, little leaps) is
recorded.
Hot plate (Panlab, Barcelona, Spain).
10 mice per group are recommended for reliable data analysis.
Formalin tests allows evaluation of chronic inflammatory pain.
There is no dedicated equipment: observation and manual scoring are used.
10 mice per group are recommended for reliable data analysis.
This procedure is used to assess sensitivity to electrical footshock, and is a critical control for procedures requiring foot-shocks (i.e, aversive learning).
Fear conditioning equipment (Coulbourn Instruments, Whitehall, USA)
10 mice per group are recommended for reliable data analysis.
