Marie-Christine Birling, lauréate médaille de Cristal CNRS 2023

Marie-Christine Birling, Cheffe Associée du Département d'Ingénérie Génétique de l'iCS , est lauréate de l’une des médailles de Cristal 2023 décernée par le CNRS, annonce l’organisme le jeudi 4 mai. Reconnaissant une carrière jalonnée de belles réussites, cette médaille récompense tout particulièrement l’apport de Marie-Christine Birling dans l’adaptation de la technologie CRISPR/Cas9 à l’édition du génome et la production de modèles animaux.

Avec Philippe André, c'est notre deuxième médaille Cristal du CNRS à l'ICS, ce qui démontre la qualité du travail de toutes nos équipes

INFRAFRONTIER: mouse model resources

iCS-MCI, is proud to continually contribute to the INFRAFRONTIER Services. take a look at our article "INFRAFRONTIER: mouse model resources for modelling human diseases" is now published in Mammalian Genome journal to catch up on the latest services and resources INFRAFRONTIER added to its portfolio

Whole Gene Humanization are now possible

We have successfully validated a method enabling the precise replacement of a mouse gene with its human (or any other species) counterpart. Diverging from prevailing platforms that insert the human fragment (usually as cDNA) into an open chromatin region such as Rosa26 or H11 locus or in the gene of interest, our approach stands out by allowing for the precise targeting of the mouse genome. This distinctive strategy not only achieves genuine humanization of the gene but also holds the distinct advantage of simultaneously knocking out the mouse gene.

Such models allow the expression of all human isoforms and all human introns will be present.

Our approach integrates a unique, in-house-derived ES cell line combined with advanced CRISPR/Cas9 technology.

To sum up:

•Entire gene replacement are now easily achievable

•Entire gene insertion is a powerful alternative to classical Rosa26 locus knockin approaches. Our approach allows insertion of a entire gene in any locus including if course in “safe harbor” locus like Rosa26 locus.

•Our strong expertise in BAC manipulation allows us not only to completely replace/insert a gene, but also to propose any additional mutations, i.e. 2-in-1 or 3-in-1 models, such as designing specific pathological variants or conditional potential by inserting loxP sequences, for example.

 

 

Good practices

Of Rodents and Men

A Dossier of the Magazine Savoir(s), University of Strasbourg
In immersion at the Institut Clinique de la Souris

Full Article