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Services

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The Genetic Engineering and Model Validation department is in charge of the generation of customized and ready-to-use genetically modified mouse.

Since its creation in 2002, more than 1300 mouse models were done for our customers: academic labs, European Union or big pharmaceutical groups.

We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129/Pas or C57BL/6N ES cells into blastocysts or by DNA pronuclear injection. 

We also focus our efforts on reducing the timelines by developing new techniques and standardizing our process. CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models. Now we can offer the generation of knock-out models though direct injection into fertilized mouse oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells. Finally, CRISPR/Cas9 genome editing opens a new area in rat genetics, and we are now able to provide researchers with rat models.To secure your project, we provide quality control procedures and develop a quality management system / assurance.

Each model can be validated at the DNA (ddPCR, Southern Blot), RNA (Northern Blot) or protein level (Immunohistochemistry, Western Blot) by our genotyping and gene expression services

Available models

Package

The development of a genetically modified mouse line by homologous recombination includes:

  • The design of a strategy for the modification of the targeted gene
  • The construction of the target vector
  • The electroporation in 129 S2/SvPas ES or in C57BL/6N cells
  • The screening of up to a maximum of 700 ES colonies and the confirmation of ES positive clones if needed
  • The development of chimeric mice
  • The establishment of the genetically modified mouse line by breeding with the chimeric mice (Germ Line Transmission (GLT) of the mutation)
  • The in vivo excision of the selection marker by breeding
  • The delivery of at least 2 heterozygous mice for the requested type of mutation (cKO, KI, ...)
  • The delivery of a technical report that detailed the main results obtained in each step and includes a genotyping protocol
  • On request: validation of the functionality of the genetic modifications introduced in vivo

The development of a transgenic mouse by micro-injection of a DNA construction into the pronucleus of fertilized eggs (PNI transgenesis) includes:

  • The design of a strategy of the vector development for the transgenic mouse model
  • The construction of the target vector
  • The micro-injection of the vector into a maximum of 300 C57BL/6N mice eggs (other backgrounds on request)
  • The genotyping of the founders and their delivery
  • On request: generation of G1 animals and qRT-PCR evaluation of expression of each line

These services are 100% customized: each step can be performed or not in our institute.

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