The Genetic Engineering and Model Validation department is in charge of the generation of customized and ready-to-use genetically modified mouse.
Since its creation in 2002, more than 1300 mouse models were done for our customers: academic labs, European Union or big pharmaceutical groups.
We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129/Pas or C57BL/6N ES cells into blastocysts or by DNA pronuclear injection.
We also focus our efforts on reducing the timelines by developing new techniques and standardizing our process. CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models. Now we can offer the generation of knock-out models though direct injection into fertilized mouse oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells. Finally, CRISPR/Cas9 genome editing opens a new area in rat genetics, and we are now able to provide researchers with rat models.To secure your project, we provide quality control procedures and develop a quality management system / assurance.
Each model can be validated at the DNA (ddPCR, Southern Blot), RNA (Northern Blot) or protein level (Immunohistochemistry, Western Blot) by our genotyping and gene expression services
We offer customized genotyping services for any mutation including knock-out, knock-in, transgenic or any other genetically modified rodent model. It is particularly useful during model creation for more in depth validation of a mutant model (QC of the mouse and rat lines). Many controls are necessary to validate complex alleles (e.g. after Flp or Cre recombination) or multiple regions of a locus.
Our service cover any genotyping step:
The development of a genetically modified mouse line by homologous recombination includes:
The development of a transgenic mouse by micro-injection of a DNA construction into the pronucleus of fertilized eggs (PNI transgenesis) includes:
These services are 100% customized: each step can be performed or not in our institute.
PHENOMIN-ICS has joined forces with Charles River, a leading scientific CRO, to deliver a complete solution for mouse and rat model creation. Our combined in vitro and in vivo expertise provides an optimum environment for creating, characterising, preserving and distributing your genetically engineered knock-out, knock-in and transgenic rodent models.
CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models, however, its impact on the genome still requires careful consideration. For further insight we are pleased to present the following Charles River-hosted webinars presented by PHENOMIN-ICS: