The Genetic Engineering and Model Validation department is in charge of generating customized and ready-to-use genetically modified mouse. It provides services and develops new tools in generation and validation of mouse model. Since its creation in 2002, more than 1300 mouse models were designed for our customers: academic labs, European Union scientists or big pharmaceutical groups.
Since 2016, CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models. Now we can offer the generation of knock-out models though direct injection into fertilized mouse oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.
Finally, CRISPR/Cas9 genome editing opens a new area in rat genetics.
24 experienced biologists are exclusively dedicated on models design, molecular biology, and cell biology and embryo manipulation.
We offer quality custom generation of a wide range of mouse models developed by injection of Sv129/Pas or C57BL/6N ES cells in blastocysts or by pronuclear injection:
Pronucleus modification :
Modification through ES cell :
Molecular Characterization :
We also focus our efforts on reducing the timelines by developing new techniques and standardizing our process. To secure your project, we provide quality control procedures and develop a quality management system / assurance. Each model can be validated at the DNA, RNA or protein level by our genotyping and gene expression services.