The Genetic Engineering and Design service provides expert advises, proposes strategies and takes care of the design of new targeting constructs. All kind of constructs can be developed in the service:
- Constructs for random insertion of a transgene (traditional or BAC construct),
- Constructs targeted insertion of a transgene, or for targeted transgenesis (Constitutive knock out, Tissue specific knock out, Point mutation, Humanization, Flex models, etc)
- The Genetic Engineering service is also in charge of the screening and validation of the ES cell clones. Long range PCR analysis, sequencing of the inserted fragment, as well as Southern blot analysis on the genomic DNA of embryonic stem (ES) cells are routinely performed.