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Objectives

This training is aimed at providing a theoretical and practical background knowledge destined for researchers and engineers that are willing to acquire primary expertise in mouse development. The topics will cover dissection of post implant embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.

Prospectives attendees

Researchers, engineers, scientists. Need to have the basic knowledge in animal biology to understand mouse development physiology and the biological technics for phenotyping. 

Content

Lectures 

• Phenotyping of mouse embryos 
• Determining the window of lethality of mutant mice in utero
• Introduction to Mouse embryonic development
• Classical histology (Fixation, embedding, orientation, sectioning and staining),
• Immunohistochemistry and in situ hybridization
• Imaging technics (HREM, micro CT, OPT)
• High frequency echography to visualize and quantify embryonic development
• The in-vitro culture of mouse embryos/ drug testing
• Confocal macroscopy: application in embryology

Workshops

• Collection and fixation of mouse embryos and placentas at E10.5 (mid gestation stage)
• Whole mount Lac Z staining of E10.5 embryos (Step1)
• Observation of embryos by echography
• Observation of embryos by micro CT, OPT (potentialy)
• Whole mount Lac Z staining of E10.5 embryos (step 2) + observation
• Collection of mouse embryos at E8.5 (early embryonic stage)
• Collection of mouse fetuses and placentas at E18.5 (just before birth)
• Observation of embryos at the confocal macroscope
• Visualization of histological sections at different stages of development. Example of abnormalities.